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Image Search Results
Journal: Clinical and Experimental Immunology
Article Title: Cultured lymphocytes’ mitochondrial genome integrity is not altered by cladribine
doi: 10.1093/cei/uxad112
Figure Lengend Snippet: Proliferation rates of the T-cell lines and effects of cladribine on cell viability. (a) The growth rate of Jurkat E6.1 is faster than the CCRF-CEM cell line ( n = 3). (b) A cladribine dose response curve in Jurkat E6.1 cell line for cell proliferation with 48 hours ( n = 5) and 96 hours culture ( n = 5). (c) The effect of cladribine on cell proliferation in Jurkat with 12 days ( n = 5) and 16 days in culture ( n = 5). (d) The effect of cladribine on cell proliferation in CCRF-CEM with 2 days ( n = 5), 8 days ( n = 4), and 12 days ( n = 4) in culture ( n = 5).
Article Snippet: The human
Techniques:
Journal: Clinical and Experimental Immunology
Article Title: Cultured lymphocytes’ mitochondrial genome integrity is not altered by cladribine
doi: 10.1093/cei/uxad112
Figure Lengend Snippet: Effect of cladribine on mitochondrial protein synthesis. (a) Immunoblot of MT-ATP6, MT-CO1, MRPL11, MRPS35, CASPASE3, SDHA, and CANX after 12 days culture with cladribine. In this experiment, 10 nM cladribine in CCRF cells resulted in extensive cell death, and immunoblot was not performed. (b) A pulse metabolic labeling of mitochondrial protein synthesis. The effect on mitochondrial protein synthesis in the CCRF-CEM and Jurkat cells after treatment with cladribine for 8 days ( n = 3) or 12 days ( n = 3).
Article Snippet: The human
Techniques: Western Blot, Labeling
Journal: Chemical Science
Article Title: Direct decarboxylation of ten-eleven translocation-produced 5-carboxylcytosine in mammalian genomes forms a new mechanism for active DNA demethylation
doi: 10.1039/d1sc02161c
Figure Lengend Snippet: Decarboxylation of F-5caC is a rapid process and occurs in diverse mammalian cells. (a–c) Quantification of F-5caC, F-dC, and F-5mC levels in genomic DNA of HEK293T cells upon feeding of 300 μM of F-5caC for different times. (d) Quantification of F-5caC, F-dC, and F-5mC levels in genomic DNA of Jurkat-T, MCF-7, and Neuro-2a cells upon feeding of 300 μM of F-5caC for 3 d. (e) Schematic illustration of the active 5mC demethylation through two pathways: the direct decarboxylation of TET-produced 5caC to cytosine in mammalian genomes established in the current study and the previous TET-TDG-BER pathway.
Article Snippet: The human embryonic kidney epithelial cell line (HEK293T),
Techniques: Produced